September 21-25,
Kyiv, Ukraine

Institute of Molecular Biology and Genetics
National Academy of Science of Ukraine

SECTIONS: Molecular Biology • Genetics • Bioinformatics and Structural Biology • Biotechnology • Physiology • Cell Biology

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Prof. Rafal Czajkowski

PhD, Nencki Institute of Experimental Biology, Warsaw (Poland)

Prof. Valdas Laurinavicius

Habil. Dr,Institute of Biochemistry, Vilnius University, Vilnius (Lithuania)

Prof. Matthias Bochtler

PhD,  International Institute of Molecular and Cell Biology, Warsaw (Poland)

Prof. Petr Svoboda

PhD, Institute of Molecular Genetics of the ASCR, Prague (Czech Republic)

Prof. Andriy Sibirny

PhD, Dr.Sc., Prof. Member of NASU, Institute of Cell Biology, National Academy of Sciences of Ukraine, Lviv (Ukraine)

Dr. Eugene Barsov

Leidos Biomedical Research, Inc., Frederick National Laboratory for Cancer Research, Frederick (USA) 

Prof. Andriy Luzhetskyy

Dr., Helmholtz-Institute for Pharmaceutical Research Saarland, Saarbrucken (Germany) 

Prof. Mikhail Gelfand

PhD, Dr.Sc., Institute for Information Transmission Problems, Russian Academy of Sciences, Moscow (Russian Federation) 


Modeling biological processes with focus on metabolism models

Institute of Molecular Biology and Genetics
National Academy of Sciences of Ukraine

Tutor: Ruslan Rodriguez

Course description:

    What you will learn?

  • Tasks that can be solved with models;
  • Types of models;
  • What type of model suits best for your study, how to choose model;
  • Pros and cons of different modeling techinques;
  • Most popular modeling software;
  • Hands-on: - Databases of models - Metabolic network reconstruction - Constraints-based modeling - Kinetic modeling - Stochastic modeling

Basics of Chromatography

Zabolotny Institute of Microbiology and Virology
National Academy of Sciences of Ukraine

Tutor: Andriy Ostapchuk

Course description:

The theoretical basis of the method of chromatography, classification and designation. Construction of gas and liquid chromatograph, methods of detection. Chromatography-mass spectrometry - especially variations of the method of mass spectrometric detectors and their purpose. Methods of ionization. Analysis of fatty acid methyl esters by gas chromatography-mass spectrometry for identifying microorganisms. Determination of caffeine by liquid chromatography.

Introduction into flow cytometry

Palladin Institute of biochemistry
National Academy of Sciences of Ukraine

Tutor: Kyrylo A. Pyrshev

Course description:

Flow cytometry is a generic name for techniques which involve the analysis and/or separation of particles in a flowing stream by quantification of optical parameters. The aim of the course is to introduce students to practical features of flow cytometry.

    The course will take you through:

  • Short introduction in flow cytometry;
  • Basic knowledge: how to design and choose the right experiment; sample preparation;
  • How to operate the instrument: overview of Beckman Coulter XL flow cytometer and software for analysis;
  • Hands-on experiments: creating the optimal protocols for scattering and fluorescent approaches for studying the cell death.

Langendorff Isolated Rat Heart

Bohomolets Institute of Physiology
National Academy of Sciences of Ukraine

Tutor: Yulia Goshovska

Course description:

Langendorff technique allow to measure contractile activity of isolated rat heart by latex balloon placed in left ventricle and connected to registrator. The participants will be able to measure the main indexes of heart function (left ventricular developed pressure, dP/dt, heart rate and coronary flow). You will learn the main principle of heart work, oxygen utilization by myocardium and regulation of heart functions those not depended from nerve control. You will be able to perform the process of heart connection to the Langendorff apparatus and pulmonary artery catheterization, measurements of oxygen tension in solutions in- and out flowing from the heart by your own.

Basic patch-clamp technique: theory and some practice

Bohomolets Institute of Physiology
National Academy of Sciences of Ukraine

Tutor: Oleksandr Dovgan

Requirements: physics (basic knowledge), good motor skills of hands.

Course description:

During 4-hours training some basic items of electrophysiology will be introduced. Composition of internal and external solutions, ‘”cooking” desired glass pipettes  , basic  interpretation of  obtained electrophysiological registrations and other technical moments  will be discussed. During practical part, we will try to obtain whole-cell patch recordings  from cultured hippocampal neurons.

Transmission (antireflection) electron microscopy: basic objectives and approaches for use

Zabolotny Institute of Microbiology and Virology
National Academy of Sciences of Ukraine

Tutor: Serge Voychuk

Course description:

  1. Introduction to the method and microscope JEM-1400 (Jeol, Japan)
    • Problems solved by TEM
    • Introduction to the structure and characteristics of the example TEM JEM-1400
  2. Methodical bases of preparation of samples for analysis by electron microscopy:
    • Preparation of formvar and carbon films
    • Preparation of samples and placement onto mesh
    • Positive and negative staining
    • Image analysis by TEM

Confocal microscopy

Bohomolets Institute of Physiology
National Academy of Sciences of Ukraine

Tutor: Oksana Rybachuk

Course description:

Immunohistochemical staining of organotypic culture of hippocampi and stem cells of different origin (neural stem cells and bone marrow stem cells).

DNA Methylation Analysis

Location: Bohomolets Institute of Physiology National Academy of Sciences of Ukraine

Tutor: Andrii Kuklin

Course description:

DNA can be modified by methylation of cytosine bases, particularly cytosines preceding guanines (CpGdinucleotides), by enzymes called DNA methyltransferases. This epigenetic modification generally functions to repress gene expression and is important for the regulation of cellular differentiation and development. DNA methylation can also serve as an epigenetic biomarker for many human diseases. Bisulfite conversion, also known as bisulfite treatment, is used to deaminate non-methylated cytosine to produce uracil in DNA. Methylated cytosines (5-methylcytosines) are protected from the conversion to uracil, allowing the use of sequencing to determine their locations at single-nucleotide resolution. Bisulfite is considered the "gold standard" for downstream applications to assess DNA methylation status.

During this workshop we will go through entire process of DNA methylation analysis starting from bisulfite conversion with following PCR and restriction analysis.


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Organizing Committee

tel.: +38 044 526 20 16

Victoria Kosach (Secretary)


Oleksandr Skorokhod (Head of the Organizing Committee)


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